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Cation of a plasmid containing the JCV origin of replication. Using an in vivo approach with permissive PHFG cells, it was shown that JCV TAg is required for DNA replication, and JCV T9 proteins contribute to replication efficiency [45]. Further, a truncated JCV tAg mutant, encoding amino acids 1?9, replicated with similar efficiency to wild type JCV at days 7 and 14 p.t., but significant inhibiti

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